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abts chromogenic solution  (MedChemExpress)


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    Structured Review

    MedChemExpress abts chromogenic solution
    Abts Chromogenic Solution, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abts+chromogenic+solution/ABTS/10__3390_slash_s26103024-71-56-60
    Average 94 stars, based on 22 article reviews
    abts chromogenic solution - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: Affinity Peptide-Based Circularly Permuted Fluorescent Protein Biosensors Loaded in a Microfluidic System for Systemic Lupus Erythematosus Diagnosis
    Article Snippet: .. Subsequently, 200 μL of horseradish peroxidase (HRP)-conjugated anti-M13 antibody (11973-MM05T-H, YQShengzhou, Beijing, China), diluted 1:5000, was added to each well and incubated at room temperature for 1 h. After additional washing, 200 μL of ABTS chromogenic solution (HY-15902, MedChemExpress LLC, Monmouth Junction, NJ 08852, USA) was added to each well and incubated at room temperature for 10–60 min. Absorbance was measured at 405–415 nm using a microplate reader to evaluate the binding of the affinity peptides to the anti-dsDNA antibody. .. Real-time detection of the binding kinetic parameters between the screened affinity peptides and the anti-dsDNA antibody was performed by means of bio-layer interferometry (BLI) using a Fortebio Octet RH96 instrument (Sartorius AG, Goettingen, Germany).

    Article Title: Affinity Peptide-Based Circularly Permuted Fluorescent Protein Biosensors Loaded in a Microfluidic System for Systemic Lupus Erythematosus Diagnosis
    Article Snippet: .. After incubation at room temperature for 1–2 h, the wells were washed six times with TBST buffer to remove unbound phages. https://doi.org/10.3390/s26103024 Subsequently, 200 μL of horseradish peroxidase (HRP)-conjugated anti-M13 antibody (11973-MM05T-H, YQShengzhou, Beijing, China), diluted 1:5000, was added to each well and incubated at room temperature for 1 h. After additional washing, 200 μL of ABTS chromogenic solution (HY-15902, MedChemExpress LLC, Monmouth Junction, NJ 08852, USA) was added to each well and incubated at room temperature for 10–60 min. Absorbance was measured at 405–415 nm using a microplate reader to evaluate the binding of the affinity peptides to the anti-dsDNA antibody. .. Real-time detection of the binding kinetic parameters between the screened affinity peptides and the anti-dsDNA antibody was performed by means of bio-layer interferometry (BLI) using a Fortebio Octet RH96 instrument (Sartorius AG, Goettingen, Germany).

    Binding Assay:

    Article Title: Affinity Peptide-Based Circularly Permuted Fluorescent Protein Biosensors Loaded in a Microfluidic System for Systemic Lupus Erythematosus Diagnosis
    Article Snippet: .. Subsequently, 200 μL of horseradish peroxidase (HRP)-conjugated anti-M13 antibody (11973-MM05T-H, YQShengzhou, Beijing, China), diluted 1:5000, was added to each well and incubated at room temperature for 1 h. After additional washing, 200 μL of ABTS chromogenic solution (HY-15902, MedChemExpress LLC, Monmouth Junction, NJ 08852, USA) was added to each well and incubated at room temperature for 10–60 min. Absorbance was measured at 405–415 nm using a microplate reader to evaluate the binding of the affinity peptides to the anti-dsDNA antibody. .. Real-time detection of the binding kinetic parameters between the screened affinity peptides and the anti-dsDNA antibody was performed by means of bio-layer interferometry (BLI) using a Fortebio Octet RH96 instrument (Sartorius AG, Goettingen, Germany).

    Article Title: Affinity Peptide-Based Circularly Permuted Fluorescent Protein Biosensors Loaded in a Microfluidic System for Systemic Lupus Erythematosus Diagnosis
    Article Snippet: .. After incubation at room temperature for 1–2 h, the wells were washed six times with TBST buffer to remove unbound phages. https://doi.org/10.3390/s26103024 Subsequently, 200 μL of horseradish peroxidase (HRP)-conjugated anti-M13 antibody (11973-MM05T-H, YQShengzhou, Beijing, China), diluted 1:5000, was added to each well and incubated at room temperature for 1 h. After additional washing, 200 μL of ABTS chromogenic solution (HY-15902, MedChemExpress LLC, Monmouth Junction, NJ 08852, USA) was added to each well and incubated at room temperature for 10–60 min. Absorbance was measured at 405–415 nm using a microplate reader to evaluate the binding of the affinity peptides to the anti-dsDNA antibody. .. Real-time detection of the binding kinetic parameters between the screened affinity peptides and the anti-dsDNA antibody was performed by means of bio-layer interferometry (BLI) using a Fortebio Octet RH96 instrument (Sartorius AG, Goettingen, Germany).



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    <t>ELISA</t> cross-reactivity of anti-S monoclonal antibodies (mAbs) (A) Graphs show antibody ELISA reactivity against S proteins of wild-type SARS-CoV-2 and its five related VOCs. The six tested ELISA antigens include S proteins of Wuhan-Hu-1 (wild-type), B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), and B.1.1.529 (Omicron). ELISA area under the curve (AUC) values were calculated for all 45 mAbs isolated from a convalescent donor with a potent serum neutralizing activity. Representative of two experiments. The names of mAbs are color coded: red, RBD-binding mAb; blue, NTD-binding mAb; green, S2-binding mAb; and black, S-ECD- but not RBD/S1/S2-binding mAb. (B) Percentage change in ELISA AUC relative to wild-type S protein. ELISA AUC results are presented as percentage of AUC normalized to the reactivity against Wuhan-Hu-1 (wild-type) S protein and are illustrated by colors: black, 0%–25%; dark gray, 25%–50%; light gray, 50%–75%; and white, >75%. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
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    Image Search Results


    ELISA cross-reactivity of anti-S monoclonal antibodies (mAbs) (A) Graphs show antibody ELISA reactivity against S proteins of wild-type SARS-CoV-2 and its five related VOCs. The six tested ELISA antigens include S proteins of Wuhan-Hu-1 (wild-type), B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), and B.1.1.529 (Omicron). ELISA area under the curve (AUC) values were calculated for all 45 mAbs isolated from a convalescent donor with a potent serum neutralizing activity. Representative of two experiments. The names of mAbs are color coded: red, RBD-binding mAb; blue, NTD-binding mAb; green, S2-binding mAb; and black, S-ECD- but not RBD/S1/S2-binding mAb. (B) Percentage change in ELISA AUC relative to wild-type S protein. ELISA AUC results are presented as percentage of AUC normalized to the reactivity against Wuhan-Hu-1 (wild-type) S protein and are illustrated by colors: black, 0%–25%; dark gray, 25%–50%; light gray, 50%–75%; and white, >75%. See also <xref ref-type=Figure S1 . " width="100%" height="100%">

    Journal: Cell Reports

    Article Title: Fortuitous somatic mutations during antibody evolution endow broad neutralization against SARS-CoV-2 Omicron variants

    doi: 10.1016/j.celrep.2023.112503

    Figure Lengend Snippet: ELISA cross-reactivity of anti-S monoclonal antibodies (mAbs) (A) Graphs show antibody ELISA reactivity against S proteins of wild-type SARS-CoV-2 and its five related VOCs. The six tested ELISA antigens include S proteins of Wuhan-Hu-1 (wild-type), B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), B.1.617.2 (Delta), and B.1.1.529 (Omicron). ELISA area under the curve (AUC) values were calculated for all 45 mAbs isolated from a convalescent donor with a potent serum neutralizing activity. Representative of two experiments. The names of mAbs are color coded: red, RBD-binding mAb; blue, NTD-binding mAb; green, S2-binding mAb; and black, S-ECD- but not RBD/S1/S2-binding mAb. (B) Percentage change in ELISA AUC relative to wild-type S protein. ELISA AUC results are presented as percentage of AUC normalized to the reactivity against Wuhan-Hu-1 (wild-type) S protein and are illustrated by colors: black, 0%–25%; dark gray, 25%–50%; light gray, 50%–75%; and white, >75%. See also Figure S1 .

    Article Snippet: ABTS Chromogen/substrate solution for ELISA , Thermo Fisher Scientific , Cat#00-2024.

    Techniques: Enzyme-linked Immunosorbent Assay, Bioprocessing, Isolation, Activity Assay, Binding Assay

    Journal: Cell Reports

    Article Title: Fortuitous somatic mutations during antibody evolution endow broad neutralization against SARS-CoV-2 Omicron variants

    doi: 10.1016/j.celrep.2023.112503

    Figure Lengend Snippet:

    Article Snippet: ABTS Chromogen/substrate solution for ELISA , Thermo Fisher Scientific , Cat#00-2024.

    Techniques: Virus, Recombinant, FLAG-tag, Variant Assay, Marker, Staining, Enzyme-linked Immunosorbent Assay, Luciferase, Cell Culture, Lysis, Plasmid Preparation, Transgenic Assay, Software, Sterility, Suction Filtration